Autopsy routine for the fetal heart in the 1st trimester of pregnancy

  • Cezary Niszczota, Department of Anatomy, Center of Biostructure Research, Medical University of Warsaw, Poland, Poland
  • Dr Adam Kolesnik, Department of Anatomy, Center of Biostructure Research, Medical University of Warsaw, Poland, Poland
  • The examination of fetal heart in the 1st trimester of pregnancy is difficult for echocardiographers as well as for pathologists. Pathologists use different approaches to visualize cardiac anatomy. Some of them prefer histological methods whereas others try to follow routine dissection method. We aimed to develop our own autopsy routine for small fetal hearts. Twenty normal and malformed fetal hearts aged from 8 until 12 weeks (heart length from 4.2 mm to 8.00 mm), fixed and unfixed, were submerged in water and dissected using microsurgical instruments. SMZ 1500 microscope, Nikon DS-Fi1 camera and NIS-Elements 3.00 software were used for observation and documentation. The chest was opened by medial sternotomy and the heart was dissected in situ and sequential segmental analysis was applied. External morphology of atrial appendages, great arteries were determined and heart measurements were done. Right atrium was opened by horizontal cut from base of appendage to orifice of inferior vena cava. Left atrium was opened between right and left pulmonary veins. Detailed anatomy of atriums, interatrial septum, pulmonary and systemic veins drainage was examined. Ventricles were opened along interventricular grooves (figure). Posterior right ventricle section was extended through the annulus of tricuspid valve to the right atrium for better visualization of its structures, e.g. right sinus venosus valve. In all cases morphology of chambers, atrioventricular and ventriculoarterial connections were possible to determine. We concluded that microautopsy of fetal heart is possible using classical pathological methods. Detailed structures are best visible when specimen is unfixed and submerged in water.